Targeted Proteomics is a very powerful technique for simultaneous determination of protein abundance for a selected set of proteins. The technique is based on proteolytic degradation of proteins and high performance liquid chromatography coupled to an MS instrument. Tandem MS experiments are automatically scheduled for a selection of predefined peptides, enabling accurate quantification of the protein concentration in the sample.
The BioMS Targeted Proteomics platform currently comprises a Fusion Orbitrap instrument and triple quadrupole instruments (Vantage, TSQ instruments), all equipped with nanoEasy HPLC systems.
Targeted Proteomics at BioMS comprises:
- Discovery experiments by use of data-dependent acquisition (DDA) in a Fusion Orbitrap for identification of proteins.
- Targeted experiments in which a selection of proteins are quantified.
In a DDA workflow, the instrument sequences as many peptides as possible during the experiment, whereas in a targeted workflow, a selection of predefined peptides are quantified.