Targeted Proteomics is a very powerful technique for simultaneous determination of protein abundance for a selected set of proteins. The technique is based on proteolytic degradation of proteins and high performance liquid chromatography coupled to an MS instrument. Tandem MS experiments are automatically scheduled for a selection of predefined peptides, enabling accurate quantification of the protein concentration in the sample.
The BioMS Targeted Proteomics platform currently comprises a Fusion Orbitrap instrument and triple quadrupole instruments (Vantage, TSQ instruments), all equipped with nanoEasy HPLC systems.
Please feel free to contact us with questions or for discussion about your targeted proteomics needs.
Targeted Proteomics at BioMS comprises:
- Discovery experiments by use of data-dependent acquisition (DDA) in a Fusion Orbitrap for identification of proteins.
- Targeted experiments in which a selection of proteins are quantified.
In a DDA workflow, the instrument sequences as many peptides as possible during the experiment, whereas in a targeted workflow, a selection of predefined peptides are quantified.